Carica papaya Leaf Extract in the In Vitro Tripneustes gratilla Embryo

This study aims to determine the antimitotic activity of the ethanolic leaf extract of C. papaya in the sea urchin (Tripneust gratilla) embryo. The inhibition of cell proliferation of the crude extract in the sea urchin embryo was observed in three different concentrations (0.5%, 1.00% and 1.5%) of the extract and two control groups. The time interval of each developmental stage of sea urchin’s embryo treated with the different concentrations of the C. papaya extract was higher compared to negative control group. Comparing the results in cleavage of the experimental groups, the lowest concentration (0.50%) of the plant extract showed the fastest mitotic activity compared to other concentrations. On the other hand, the highest concentration (1.50%) showed the slowest embryonic development compared to other treatment concentrations. In addition, 0.50% concentration showed a comparable result cell stages. C. papaya leaf extract showed antimitotic activity in the sea urchin embryos. The inhibition of sea urchin’s proliferation in each developmental stage is dependent on the incr inhibition of the various concentrations of the plant extract and the control group is also significantly different at 0.5 le significant. Tukey pairwise comparison test result showed that most o difference between the time intervals of mitotic activity. In addition, the increasing concentration of the plant extract increased the time interval between developmental stages. phytochemical component present in the plant. Previous studies revealed that phytochemical analysis of C. papaya contained saponins, cardiac glycosides, and alkaloids. The phytochemical component of C. papaya is a goo suitable for a better pharmacological feature and a potent anti